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Application of RNAi technology and fluorescent protein markers to study membrane traffic in Caenorhabditis elegans

Poteryaev, Dmitry and Spang, Anne. (2008) Application of RNAi technology and fluorescent protein markers to study membrane traffic in Caenorhabditis elegans. Methods in Molecular Biology, 440. pp. 331-347.

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Official URL: http://edoc.unibas.ch/dok/A5259736

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Abstract

Ribonucleic acid interference (RNAi) is a powerful tool for study of the intracellular membrane transport and membrane organelle behavior in the nematode Caenorhabditis elegans. This model organism has gained popularity in the trafficking field because of its relative simplicity, yet multicellularity. Caenorhabditis elegans is fully sequenced and has an annotated genome, it is easy to maintain, and a growing number of transgenic strains bearing markers for different membrane compartments are available. Caenorhabditis elegans is particularly well suited for protein downregulation by RNAi because of the simple but efficient methods of double-stranded RNA (dsRNA) delivery. The phenomenon of systemic RNAi in the worm further facilitates this approach. In this chapter, we describe methods and applications of RNAi in the field of membrane traffic. We summarize the fluorescent markers used as a readout for the effects of gene knockdown in different cells and tissues and give details for data acquisition and analysis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang)
UniBasel Contributors:Spang, Anne
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Humana Press
ISSN:1064-3745
e-ISSN:1940-6029
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:16 Nov 2017 15:58
Deposited On:22 Mar 2012 13:59

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