Schenk, Andreas D. and Castaño-Díez, Daniel and Gipson, Bryant and Arheit, Marcel and Zeng, Xiangyan and Stahlberg, Henning. (2010) 3D reconstruction from 2D crystal image and diffraction data. Methods in enzymology, Vol. 482. pp. 101-129.
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Official URL: http://edoc.unibas.ch/dok/A5842539
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Abstract
Electron crystallography of 2D protein crystals can determine the structure of membrane embedded proteins at high resolution. Images or electron diffraction patterns are recorded with the electron microscope of the frozen hydrated samples, and the 3D structure of the proteins is then determined by computer data processing. Here we introduce the image-processing algorithms for crystallographic Fourier space based methods using the Medical Research Council (MRC) programs, and illustrate the usage of the software packages 2dx, XDP, and IPLT.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg) |
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| UniBasel Contributors: | Stahlberg, Henning and Castano Diez, Daniel and Gipson, Bryant and Arheit, Marcel |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Elsevier |
| ISSN: | 0076-6879 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
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| Last Modified: | 14 Sep 2012 07:17 |
| Deposited On: | 14 Sep 2012 06:38 |
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