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Preparation of 2D crystals of membrane proteins for high-resolution electron crystallography data collection

Abeyrathne, Priyanka D. and Chami, Mohamed and Pantelic, Radosav S. and Goldie, Kenneth N. and Stahlberg, Henning. (2010) Preparation of 2D crystals of membrane proteins for high-resolution electron crystallography data collection. Methods in enzymology, Vol. 481. pp. 25-43.

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Official URL: http://edoc.unibas.ch/dok/A5842540

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Abstract

Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscopy at close to native conditions. In this review, we discuss the factors of sample preparation that are key to elucidating the atomic structure of membrane proteins using electron crystallography.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0076-6879
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Sep 2012 07:17
Deposited On:14 Sep 2012 06:38

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