Duthaler, U. and Rinaldi, L. and Maurelli, M. P. and Vargas, M. and Utzinger, J. and Cringoli, G. and Keiser, J.. (2010) Fasciola hepatica: comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats. Experimental parasitology : EP, Vol. 126, H. 2. pp. 161-166.
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Official URL: http://edoc.unibas.ch/dok/A5842896
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Abstract
We compared the sedimentation and FLOTAC techniques for the detection and quantification of Fasciola hepatica eggs in faecal samples obtained from 120 experimentally-infected rats before intervention, and in 42 rats after drug administration. Additionally, the average time for a single test was determined. A single FLOTAC showed a higher sensitivity (92.6%) than 2, 4 and 8 sedimentation readings (63.0-85.2%) for detecting F. hepatica eggs in rat faeces post-treatment. On average, it took 21min to prepare and examine a single FLOTAC, whereas 114min were needed for the sedimentation method including the reading of 8 slides. In both treated and untreated rats, the sedimentation method resulted in higher mean faecal egg counts (FECs) than FLOTAC (P>0.05). In view of the high sensitivity and efficiency, the FLOTAC technique holds promise for experimental work in the F. hepatica-rat model. Additional research is needed to determine the reasons for the observed differences in FECs
Faculties and Departments: | 03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie 03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie 09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Helminth Drug Development (Keiser) |
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UniBasel Contributors: | Duthaler, Urs and Keiser, Jennifer and Utzinger, Jürg |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Academic Press |
ISSN: | 0014-4894 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Related URLs: | |
Identification Number: |
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Last Modified: | 24 May 2013 09:10 |
Deposited On: | 14 Sep 2012 06:53 |
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