Krähenbühl, Barbara and Hiller, Sebastian and Wider, Gerhard. (2011) 4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins. Journal of biomolecular NMR, Vol. 51. pp. 313-318.
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Official URL: http://edoc.unibas.ch/dok/A6001520
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Abstract
A four-dimensional (4D) APSY (automated projection spectroscopy)-HBCB(CG)CDHD experiment is presented. This 4D experiment correlates aromatic with aliphatic carbon and proton resonances from the same amino acid side chain of proteins in aqueous solution. It thus allows unambiguous sequence-specific assignment of aromatic amino acid ring signals based on backbone assignments. Compared to conventional 2D approaches, the inclusion of evolution periods on (1)H(?) and (13)C(?) efficiently removes overlaps, and provides two additional frequencies for consequent automated or manual matching. The experiment was successfully applied to three proteins with molecular weights from 6 to 13 kDa. For the complementation of the assignment of the aromatic resonances, TOCSY- or COSY-based versions of a 4D APSY-HCCH(aro) sequence are proposed.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Hiller) |
|---|---|
| UniBasel Contributors: | Hiller Odermatt, Sebastian |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Springer |
| ISSN: | 0925-2738 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
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| Last Modified: | 13 Dec 2017 11:46 |
| Deposited On: | 11 Oct 2012 15:18 |
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