Gong, Xiao Yang and Dobrunz, Dorninik and Kumin, Michael and Wiesner, Markus and Revell, Jefferson D. and Wennemers, Helma and Hauser, Peter C.. (2008) Separating stereoisomers of di-, tri-, and tetrapeptides using capillary electrophoresis with contactless conductivity detection. Journal of Separation Science, 31 (3). pp. 565-573.
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Official URL: http://edoc.unibas.ch/dok/A6001385
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Abstract
The separation and detection of small oligopeptides in CE with contactless conductivity detection were demonstrated. A strongly acidic separation buffer (0.5 M acetic acid) was employed in order to render the species cationic. Separation of the stereoisomers was achieved in typically 10-15 min by using either dimethyl-beta-CD (DM-beta-CD), (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18C(6)H(4)), a combination of the two substances, or of histidine, as buffer additives. Calibration curves were determined for isomers of Gly-Asp and H-Pro-Asp-NH2, in the range of 0.05-0.5 mM and 0.1-1 mM, respectively, and were found to be linear. LODs were determined to be in the order of 1.0 mu M. The determination of isomeric impurities down to about 1 also be successfully determined on an electrophoretic lab-on-chip device, with analysis times of a few minutes.
Faculties and Departments: | 05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser) |
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UniBasel Contributors: | Hauser, Peter C. P. C. and Gong, Xiaoyang and Dobrunz, Dominik and Wiesner, Markus and Wennemers, Helma |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Wiley |
ISSN: | 1615-9306 |
e-ISSN: | 1615-9314 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Identification Number: |
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Last Modified: | 29 Mar 2017 13:21 |
Deposited On: | 11 Oct 2012 15:25 |
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