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Separating stereoisomers of di-, tri-, and tetrapeptides using capillary electrophoresis with contactless conductivity detection

Gong, Xiao Yang and Dobrunz, Dorninik and Kumin, Michael and Wiesner, Markus and Revell, Jefferson D. and Wennemers, Helma and Hauser, Peter C.. (2008) Separating stereoisomers of di-, tri-, and tetrapeptides using capillary electrophoresis with contactless conductivity detection. Journal of Separation Science, 31 (3). pp. 565-573.

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Official URL: http://edoc.unibas.ch/dok/A6001385

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Abstract

The separation and detection of small oligopeptides in CE with contactless conductivity detection were demonstrated. A strongly acidic separation buffer (0.5 M acetic acid) was employed in order to render the species cationic. Separation of the stereoisomers was achieved in typically 10-15 min by using either dimethyl-beta-CD (DM-beta-CD), (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18C(6)H(4)), a combination of the two substances, or of histidine, as buffer additives. Calibration curves were determined for isomers of Gly-Asp and H-Pro-Asp-NH2, in the range of 0.05-0.5 mM and 0.1-1 mM, respectively, and were found to be linear. LODs were determined to be in the order of 1.0 mu M. The determination of isomeric impurities down to about 1 also be successfully determined on an electrophoretic lab-on-chip device, with analysis times of a few minutes.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C. and Gong, Xiaoyang and Dobrunz, Dominik and Wiesner, Markus and Wennemers, Helma
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1615-9306
e-ISSN:1615-9314
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:29 Mar 2017 13:21
Deposited On:11 Oct 2012 15:25

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