Braun, T. and Backmann, N. and Vogtli, M. and Bietsch, A. and Engel, A. and Lang, H. -P. and Gerber, C. and Hegner, M.. (2006) Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors. Biophysical journal, Vol. 90, H. 8. pp. 2970-2977.
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Official URL: http://edoc.unibas.ch/dok/A5257631
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Abstract
Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern: 1), reliable functionalization of micromechanical cantilever arrays with proteoliposomes using ink jet spotting; 2), successful detection of the prosthetic retinal removal (bleaching) from the bacteriorhodopsin protein by measuring the induced nanomechanical surface stress change; and 3), the quantitative response thereof, which depends linearly on the amount of removed retinal. Our results show this technique to be a potential tool to measure membrane protein-based receptor-ligand interactions and conformational changes.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel) |
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UniBasel Contributors: | Engel, Andreas H |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Biophysical Society |
ISSN: | 0006-3495 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Last Modified: | 22 Mar 2012 14:20 |
Deposited On: | 22 Mar 2012 13:18 |
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