Protein traffic in intestinal epithelial cells

Cell culture systems, in particular Caco-2, and sucrase-isomaltase deficiency in humans are attractive models to study exocytic protein traffic in absorptive intestinal epithelial cells. Transport from ER to and through the Golgi is asynchronous and may depend on protein folding rather than oligomerization. Apical and basolateral proteins are sorted both intracellularly and from the basolateral membrane. A model is presented for the sorting of apical and basolateral proteins. Brush border proteins in lysosomes mainly originate from the Golgi and may reflect a regulatory or quality control mechanism. Apical transport and transcytosis but not basolateral transport are facilitated by microtubules.