Arheit, Marcel and Castaño-Díez, Daniel and Thierry, Raphaël and Gipson, Bryant R. and Zeng, Xiangyan and Stahlberg, Henning. (2013) Image Processing of 2D Crystal Images. Methods in Molecular Biology, 955. pp. 171-194.
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Official URL: http://edoc.unibas.ch/dok/A6056066
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Abstract
Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to image frozen-hydrated 2D crystals. The processing of recorded images exploits the periodic arrangement of the structures in the images to extract the amplitudes and phases of diffraction spots in Fourier space. However, image imperfections require a crystal unbending procedure to be applied to the image before evaluation in Fourier space. We here describe the process of 2D crystal image unbending, using the 2dx software system.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg) |
|---|---|
| UniBasel Contributors: | Stahlberg, Henning |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Humana Press |
| ISSN: | 1064-3745 |
| e-ISSN: | 1940-6029 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
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| Identification Number: |
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| Last Modified: | 12 Oct 2017 10:05 |
| Deposited On: | 04 Jan 2013 08:37 |
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