Functional hierarchy of AUUUA motifs in mediating rapid interleukin-3 mRNA decay

In mast cells, expression of interleukin-3 (IL-3) is induced following IgE-receptor activation via calcium-dependent mRNA stabilization. We performed a mutational analysis of the 8 AUUUA motifs located in the 3'-untranslated region of IL-3 mRNA, and analyzed the effect on mRNA stability in PB-3c mast cells. Similar to endogenous IL-3 mRNA, exogenous IL-3 transcripts had a short half-life and were stabilized following stimulation of calcium influx by ionomycin. Specific mutation of 3 AUUUA motifs had almost the same stabilizing effect as deletion of the entire AU-rich region. Mutating even a single critical motif led to a weak, but significant mRNA stabilization. Ionomycin treatment did not further enhance the expression of mutationally stabilized transcripts. Our data fit a model, where within a cluster of 6 AUUUA motifs, 3 adjacent motifs are required for binding of a factor which mediates rapid IL-3 mRNA decay. Activity of such a factor might be under control of a calcium-dependent pathway.