Keller, W.. (1972) RNA-primed DNA synthesis in vitro. Proceedings of the National Academy of Sciences of the United States of America, 69 (6). pp. 1560-1564.
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Official URL: http://edoc.unibas.ch/dok/A5258020
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Abstract
In vitro DNA synthesis on single-stranded circular DNA can be initiated by RNA primers. RNA chains are covalently extended by DNA polymerase II from KB cells and DNA polymerase I from Micrococcus luteus, but not by an RNA-dependent DNA polymerase from avian myeloblastosis virus. The reaction product consists of DNA chains with a piece of RNA at their 5'-ends, hydrogen bonded to the template DNA. The primer RNA is linked to the product DNA via a 3':5'-phosphodiester bond, and can be specifically removed by ribonuclease H. The possible role of ribonuclease H in RNA-primed DNA synthesis in vivo is discussed.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Keller) |
|---|---|
| UniBasel Contributors: | Keller, Walter |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | National Academy of Sciences |
| ISSN: | 0027-8424 |
| e-ISSN: | 1091-6490 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Related URLs: | |
| Identification Number: |
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| Last Modified: | 07 Nov 2017 12:01 |
| Deposited On: | 22 Mar 2012 13:19 |
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