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Heat-induced gene positioning in "Caenorhabdidtis elegans" : "hsp-16" promoter drives transcription-dependent nuclear pore association

Rohner, Sabine. Heat-induced gene positioning in "Caenorhabdidtis elegans" : "hsp-16" promoter drives transcription-dependent nuclear pore association. 2013, Doctoral Thesis, University of Basel, Faculty of Science.

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Official URL: http://edoc.unibas.ch/diss/DissB_10369

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Abstract

The DNA of eukaryotic cells is packaged into transcriptionally active euchromatin and repressed heterochromatin. These two chromatin types are non-randomly distributed within the nucleus. Indeed, at the nuclear periphery, heterochromatin is enriched near the nuclear lamina whereas euchromatin is found in the vicinity of nuclear pores. Interestingly, in budding yeast, several stress inducible genes interact with nuclear pores upon activation, although the relevance of this in higher eukaryotes has been unclear. In this thesis I characterise the spatial distribution of a heat shock gene (hsp16.2) in embryos of the nematode worm Caenorhabditis elegans. I find that hsp-16.2 is preferencially found at the nuclear periphery, where it interacts with the nuclear pore complex. Using two different types of ectopically integrated hsp-16.2 promoters, I investigated the molecular mechanism of the pore-gene interaction. I find that both types of transgenes show the same distribution, even though to a lesser extent. The results presented here shed light on the promoter elements and proteins essential for the perinuclear gene localisation and suggest conservation of gene-pore interactions in yeast, worm and fly.
Advisors:Gasser, Susan
Committee Members:Ahringer, Julie
Faculties and Departments:03 Faculty of Medicine > Bereich Operative Fächer (Klinik) > Innere Organe > Urologie Kliniken BL (Gasser)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Operative Fächer (Klinik) > Innere Organe > Urologie Kliniken BL (Gasser)
UniBasel Contributors:Gasser, Susan
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:10369
Thesis status:Complete
Number of Pages:63 S.
Language:English
Identification Number:
edoc DOI:
Last Modified:02 Aug 2021 15:09
Deposited On:06 May 2013 15:03

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