Osterwalder, Marco and Galli, Antonella and Rosen, Barry and Skarnes, William C. and Zeller, Rolf and Lopez-Rios, Javier. (2010) Dual RMCE for efficient re-engineering of mouse mutant alleles. Nature methods, Vol. 7, H. 11. pp. 893-895.
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Official URL: http://edoc.unibas.ch/dok/A6004287
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Abstract
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
| Faculties and Departments: | 03 Faculty of Medicine > Departement Biomedizin > Division of Anatomy > Developmental Genetics (Zeller/Zuniga) |
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| UniBasel Contributors: | Zeller, Rolf |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Nature Publishing Group |
| ISSN: | 1548-7091 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
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| Last Modified: | 19 Jul 2013 07:43 |
| Deposited On: | 19 Jul 2013 07:35 |
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