Koegel, Sally and Ait Lahmidi, Nassima and Arnould, Christine and Chatagnier, Odile and Walder, Florian and Ineichen, Kurt and Boller, Thomas and Wipf, Daniel and Wiemken, Andres and Courty, Pierre-Emmanuel. (2013) The family of ammonium transporters (AMT) in Sorghum bicolor : two AMT members are induced locally, but not systemically in roots colonized by arbuscular mycorrhizal fungi. The new phytologist, Vol. 198, H. 3. pp. 853-865.
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Official URL: http://edoc.unibas.ch/dok/A6164895
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Abstract
Arbuscular mycorrhizal (AM) fungi contribute to plant nitrogen (N) acquisition. Recent studies demonstrated the transport of N in the form of ammonium during AM symbiosis. Here, we hypothesize that induction of specific ammonium transporter (AMT) genes in Sorghum bicolor during AM colonization might play a key role in the functionality of the symbiosis. For the first time, combining a split-root experiment and microdissection technology, we were able to assess the precise expression pattern of two AM-inducible AMTs, SbAMT3;1 and SbAMT4. Immunolocalization was used to localize the protein of SbAMT3;1. The expression of SbAMT3;1 and SbAMT4 was greatly induced locally in root cells containing arbuscules and in adjacent cells. However, a split-root experiment revealed that this induction was not systemic. By contrast, a strictly AM-induced phosphate transporter (SbPt11) was expressed systemically in the split-root experiment. However, a gradient of expression was apparent. Immunolocalization analyses demonstrated that SbAMT3;1 was present only in cells containing developing arbuscules. Our results show that the SbAMT3;1 and SbAMT4 genes are expressed in root cortical cells, which makes them ready to accommodate arbuscules, a process of considerable importance in view of the short life span of arbuscules. Additionally, SbAMT3;1 might play an important role in N transfer during AM symbiosis.
Faculties and Departments: | 05 Faculty of Science > Departement Umweltwissenschaften > Ehemalige Einheiten Umweltwissenschaften > Pflanzenphysiologie Pathogenabwehr (Boller) |
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UniBasel Contributors: | Koegel, Sally and Wiemken, Andres M. and Courty, Pierre-Emmanuel and Boller, Thomas |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Blackwell Science |
ISSN: | 0028-646X |
Note: | Publication type according to Uni Basel Research Database: Journal article |
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Identification Number: |
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Last Modified: | 13 Sep 2013 07:59 |
Deposited On: | 13 Sep 2013 07:52 |
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