Bumann, D. and Oesterhelt, D.. (1994) Purification and characterization of oxygen-evolving photosystem II core complexes from the green alga Chlamydomonas reinhardtii. Biochemistry, 33 (36). pp. 10906-10910.
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Official URL: http://edoc.unibas.ch/dok/A5259836
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Abstract
Oxygen-evolving photosystem II complexes were isolated from the green alga Chlamydomonas reinhardtii by selective solubilization of thylakoid membranes with dodecyl maltoside followed by density gradient centrifugation and anion-exchange chromatography. In the presence of CaCl2 and K3[Fe(CN)6] the complexes evolved oxygen at rates exceeding 1000 mumol (mg of chl)-1 h-1. The particles contained 40 chlorophylls a and had properties very similar to those of PSII isolated from higher plants. Chlamydomonas reinhardtii is now the first organism which can be used for both site-directed mutagenesis and detailed biochemical and biophysical characterization of oxygen-evolving photosystem II. It seems therefore to be an ideal model organism for investigation of structure-function relationships in photosynthetic oxygen evolution.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann) |
|---|---|
| UniBasel Contributors: | Bumann, Dirk |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | American Chemical Society |
| ISSN: | 0006-2960 |
| e-ISSN: | 1520-4995 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Identification Number: |
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| Last Modified: | 27 Nov 2017 07:19 |
| Deposited On: | 22 Mar 2012 13:23 |
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