Takeuchi, Koh and Frueh, Dominique P. and Sun, Zhen-Yu J. and Hiller, Sebastian and Wagner, Gerhard. (2010) CACA-TOCSY with alternate 13C-12C labeling: a 13Calpha direct detection experiment for mainchain resonance assignment, dihedral angle information, and amino acid type identification. Journal of biomolecular NMR, 47 (1). pp. 55-63.
Full text not available from this repository.
Official URL: http://edoc.unibas.ch/41057/
Downloads: Statistics Overview
Abstract
We present a (13)C direct detection CACA-TOCSY experiment for samples with alternate (13)C-(12)C labeling. It provides inter-residue correlations between (13)C(alpha) resonances of residue i and adjacent C(alpha)s at positions i - 1 and i + 1. Furthermore, longer mixing times yield correlations to C(alpha) nuclei separated by more than one residue. The experiment also provides C(alpha)-to-sidechain correlations, some amino acid type identifications and estimates for psi dihedral angles. The power of the experiment derives from the alternate (13)C-(12)C labeling with [1,3-(13)C] glycerol or [2-(13)C] glycerol, which allows utilizing the small scalar (3)J(CC) couplings that are masked by strong (1)J(CC) couplings in uniformly (13)C labeled samples.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Hiller) |
|---|---|
| UniBasel Contributors: | Hiller Odermatt, Sebastian |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Springer |
| ISSN: | 0925-2738 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Identification Number: |
|
| Last Modified: | 03 May 2019 14:47 |
| Deposited On: | 17 Aug 2016 06:40 |
Repository Staff Only: item control page