Redli, Patrick M. and Gasic, Ivana and Meraldi, Patrick and Nigg, Erich A. and Santamaria, Anna. (2016) The Ska complex promotes Aurora B activity to ensure chromosome biorientation. The Journal of Cell Biology, 215 (1). pp. 77-93.
Full text not available from this repository.
Official URL: http://edoc.unibas.ch/44403/
Downloads: Statistics Overview
Abstract
Chromosome biorientation and accurate segregation rely on the plasticity of kinetochore-microtubule (KT-MT) attachments. Aurora B facilitates KT-MT dynamics by phosphorylating kinetochore proteins that are critical for KT-MT interactions. Among the substrates whose microtubule and kinetochore binding is curtailed by Aurora B is the spindle and kinetochore-associated (Ska) complex, a key factor for KT-MT stability. Here, we show that Ska is not only a substrate of Aurora B, but is also required for Aurora B activity. Ska-deficient cells fail to biorient and display chromosome segregation errors underlying suppressed KT-MT turnover. These defects coincide with KNL1-Mis12-Ndc80 network hypophosphorylation, reduced mitotic centromere-associated kinesin localization, and Aurora B T-loop phosphorylation at kinetochores. We further show that Ska requires its microtubule-binding capability to promote Aurora B activity in cells and stimulates Aurora B catalytic activity in vitro. Finally, we show that protein phosphatase 1 counteracts Aurora B activity to enable Ska kinetochore accumulation once biorientation is achieved. We propose that Ska promotes Aurora B activity to limit its own microtubule and kinetochore association and to ensure that KT-MT dynamics and stability fall within an optimal balance for biorientation.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg) |
---|---|
UniBasel Contributors: | Nigg, Erich A. |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Rockefeller University Press |
ISSN: | 0021-9525 |
e-ISSN: | 1540-8140 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Identification Number: |
|
Last Modified: | 25 Oct 2017 14:25 |
Deposited On: | 25 Oct 2017 14:25 |
Repository Staff Only: item control page