edoc-vmtest

Proteome organization in a genome-reduced bacterium

Kühner, Sebastian and van Noort, Vera and Betts, Matthew J. and Leo-Macias, Alejandra and Batisse, Claire and Rode, Michaela and Yamada, Takuji and Maier, Tobias and Bader, Samuel and Beltran-Alvarez, Pedro and Castaño-Diez, Daniel and Chen, Wei-Hua and Devos, Damien and Güell, Marc and Norambuena, Tomas and Racke, Ines and Rybin, Vladimir and Schmidt, Alexander and Yus, Eva and Aebersold, Ruedi and Herrmann, Richard and Böttcher, Bettina and Frangakis, Achilleas S. and Russell, Robert B. and Serrano, Luis and Bork, Peer and Gavin, Anne-Claude. (2009) Proteome organization in a genome-reduced bacterium. Science, 326 (5957). pp. 1235-1240.

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Official URL: http://edoc.unibas.ch/45905/

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Abstract

The genome of Mycoplasma pneumoniae is among the smallest found in self-replicating organisms. To study the basic principles of bacterial proteome organization, we used tandem affinity purification-mass spectrometry (TAP-MS) in a proteome-wide screen. The analysis revealed 62 homomultimeric and 116 heteromultimeric soluble protein complexes, of which the majority are novel. About a third of the heteromultimeric complexes show higher levels of proteome organization, including assembly into larger, multiprotein complex entities, suggesting sequential steps in biological processes, and extensive sharing of components, implying protein multifunctionality. Incorporation of structural models for 484 proteins, single-particle electron microscopy, and cellular electron tomograms provided supporting structural details for this proteome organization. The data set provides a blueprint of the minimal cellular machinery required for life.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Association for the Advancement of Science
ISSN:0036-8075
e-ISSN:1095-9203
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:29 Nov 2017 08:16
Deposited On:29 Nov 2017 08:16

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