Riemer, Jan and Hansen, Henning Gram and Appenzeller-Herzog, Christian and Johansson, Linda and Ellgaard, Lars. (2011) Identification of the PDI-family member ERp90 as an interaction partner of ERFAD. PLoS One, 6 (2). e17037.
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Official URL: http://edoc.unibas.ch/47115/
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Abstract
In the endoplasmic reticulum (ER), members of the protein disulfide isomerase (PDI) family perform critical functions during protein maturation. Herein, we identify the previously uncharacterized PDI-family member ERp90. In cultured human cells, we find ERp90 to be a soluble ER-luminal glycoprotein that comprises five potential thioredoxin (Trx)-like domains. Mature ERp90 contains 10 cysteine residues, of which at least some form intramolecular disulfides. While none of the Trx domains contain a canonical Cys-Xaa-Xaa-Cys active-site motif, other conserved cysteines could endow the protein with redox activity. Importantly, we show that ERp90 co-immunoprecipitates with ERFAD, a flavoprotein involved in ER-associated degradation (ERAD), through what is most likely a direct interaction. We propose that the function of ERp90 is related to substrate recruitment or delivery to the ERAD retrotranslocation machinery by ERFAD.
Faculties and Departments: | 05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Molecular and Systems Toxicology (Odermatt) |
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UniBasel Contributors: | Appenzeller, Christian |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Public Library of Science |
e-ISSN: | 1932-6203 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Identification Number: | |
Last Modified: | 18 Jan 2018 10:27 |
Deposited On: | 18 Jan 2018 10:27 |
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