Schoeler, Constantin and Verdorfer, Tobias and Gaub, Hermann E. and Nash, Michael A.. (2016) Biasing effects of receptor-ligand complexes on protein-unfolding statistics. Physical review E, 94 (4). 042412.
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Official URL: http://edoc.unibas.ch/51755/
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Abstract
Protein receptor-ligand pairs are increasingly used as specific molecular handles in single-molecule protein-unfolding experiments. Further, known marker domains, also referred to as fingerprints, provide unique unfolding signatures to identify specific single-molecule interactions, when receptor-ligand pairs themselves are investigated. We show here that in cases where there is an overlap between the probability distribution associated with fingerprint domain unfolding and that associated with receptor-ligand dissociation, the experimentally measured force distributions are mutually biased. This biasing effect masks the true parameters of the underlying free energy landscape. To address this, we present a model-free theoretical framework that corrects for the biasing effect caused by such overlapping distributions.
Faculties and Departments: | 05 Faculty of Science > Departement Chemie > Chemie > Synthetic Systems (Nash) |
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UniBasel Contributors: | Nash, Michael |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | American Physical Society |
ISSN: | 2470-0045 |
e-ISSN: | 2470-0053 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: | |
edoc DOI: | |
Last Modified: | 14 Jun 2017 07:50 |
Deposited On: | 14 Jun 2017 07:45 |
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