Gauss, R. and Trautwein, M. and Sommer, T. and Spang, A.. (2005) New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae. Yeast, 22 (1). pp. 1-12.
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Official URL: http://edoc.unibas.ch/dok/A5259747
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Abstract
Epitope tagging is a powerful method for the rapid analysis of protein function. In Saccharomyces cerevisiae epitope tags are introduced easily into chromosomal loci by homologous recombination using a simple PCR-based strategy. Although quite a number of tools exist for C-terminal tagging as well as N-terminal tagging of proteins expressed by heterologous promoters, there are only very limited possibilities to tag proteins at the N-terminus and retain the endogenous expression level. Furthermore, no PCR-templates for internal tagging have been reported. Here we describe new modules that are suitable for both the repeated N-terminal and internal tagging of proteins, leaving their endogenous promoters intact. The tags include 6xHA, 9xMyc, yEGFP, TEV-GST-6xHIS, ProtA, TEV-ProtA and TEV-ProtA-7xHIS in conjunction with different heterologous selection markers.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang) |
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UniBasel Contributors: | Spang, Anne |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Wiley |
ISSN: | 0749-503X |
e-ISSN: | 1097-0061 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Identification Number: |
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Last Modified: | 16 Nov 2017 15:47 |
Deposited On: | 22 Mar 2012 13:31 |
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