Badouel, Caroline and Körner, Roman and Frank-Vaillant, Marie and Couturier, Anne and Nigg, Erich A. and Tassan, Jean-Pierre. (2006) M-phase MELK activity is regulated by MPF and MAPK. Cell cycle, Vol. 5, no. 8. pp. 883-889.
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Official URL: http://edoc.unibas.ch/dok/A5249354
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Abstract
The protein kinase MELK is implicated in the control of cell proliferation, cell cycle and mRNA splicing. We previously showed that MELK activity is correlated with its phosphorylation level, is cell cycle dependent, and maximal during mitosis. Here we report on the identification of T414, T449, T451, T481 and S498 as residues phosphorylated in Xenopus MELK (xMELK) in M-phase egg extract. Phosphorylations of T449, T451, T481 are specifically detected during mitosis. Results obtained in vivo showed that MPF and MAPK pathways are involved in xMELK phosphorylation. In vitro, MPF and MAPK directly phosphorylate xMELK and MPF phosphorylates xMELK on T481. In addition, phosphorylation by MPF and MAPK enhances MELK activity in vitro. Taken together our results indicate that MELK phosphorylation by MPF and MAPK enhance its activity during M-phase.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg) |
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| UniBasel Contributors: | Nigg, Erich A. |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Landes Bioscience |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
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| Last Modified: | 04 Sep 2015 14:31 |
| Deposited On: | 22 Mar 2012 13:49 |
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