edoc-vmtest

Targeting of E. coli beta-galactosidase to the nucleus in yeast

Hall, M. N. and Hereford, L. and Herskowitz, I.. (1984) Targeting of E. coli beta-galactosidase to the nucleus in yeast. Cell, 36 (4). pp. 1057-1065.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5258210

Downloads: Statistics Overview

Abstract

In order to identify determinants governing nuclear protein localization, we constructed a set of hybrid genes by fusing the S. cerevisiae gene, MAT alpha 2, coding for a presumptive nuclear protein, and the E. coli gene, lacZ, coding for beta-galactosidase. The resultant hybrid proteins contain 3, 13, 25, 67, or all 210 amino acids of wild-type alpha 2 protein at the amino terminus and a constant, enzymatically active portion of beta-galactosidase at the carboxy terminus. Indirect immunofluorescence and subcellular fractionation studies with yeast cells containing the alpha 2-LacZ hybrid proteins indicate that the alpha 2 segment can direct localization of beta-galactosidase to the nucleus. A segment as small as 13 amino acids from alpha 2 is sufficient for this localization. Comparison of amino acid sequences of other nuclear proteins with this region of alpha 2 reveals a sequence that may be necessary for nuclear targeting. Production of some alpha 2-LacZ hybrid proteins causes cell death, perhaps as a result of improper or incomplete localization. These studies also indicate that the alpha 2 protein, argued on genetic grounds to be a negative regulator, acts in the yeast nucleus.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hall)
UniBasel Contributors:Hall, Michael N.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
ISSN:0092-8674
e-ISSN:1097-4172
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:09 Nov 2017 08:02
Deposited On:22 Mar 2012 13:19

Repository Staff Only: item control page