Fiorito, Francesco and Hiller, Sebastian and Wider, Gerhard and Wüthrich, Kurt. (2006) Automated resonance assignment of proteins: 6D APSY-NMR. Journal of biomolecular NMR, 35 (1). pp. 27-37.
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Official URL: http://edoc.unibas.ch/41063/
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Abstract
The 6-dimensional (6D) APSY-seq-HNCOCANH NMR experiment correlates two sequentially neighboring amide moieties in proteins via the C` and Calpha nuclei, with efficient suppression of the back transfer from Calpha to the originating amide moiety. The automatic analysis of two-dimensional (2D) projections of this 6D experiment with the use of GAPRO (Hiller et al., 2005) provides a high-precision 6D peak list, which permits automated sequential assignments of proteins with the assignment software GARANT (Bartels et al., 1997). The procedure was applied to two proteins, the 63-residue 434-repressor(1-63) and the 115-residue TM1290. For both proteins, complete sequential assignments for all NMR-observable backbone resonances were obtained, and the polypeptide segments thus identified could be unambiguously located in the amino acid sequence. These results demonstrate that APSY-NMR spectroscopy in combination with a suitable assignment algorithm can provide fully automated sequence-specific backbone assignments of small proteins.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Hiller) |
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UniBasel Contributors: | Hiller Odermatt, Sebastian |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Springer |
ISSN: | 0925-2738 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: |
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edoc DOI: | |
Last Modified: | 07 Feb 2018 10:04 |
Deposited On: | 17 Aug 2016 07:23 |
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